Dioxygenase enzymes: catalytic mechanisms and chemical models
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منابع مشابه
Exploring the mechanism of tryptophan 2,3-dioxygenase
The haem proteins TDO (tryptophan 2,3-dioxygenase) and IDO (indoleamine 2,3-dioxygenase) are specific and powerful oxidation catalysts that insert one molecule of dioxygen into L-tryptophan in the first and rate-limiting step in the kynurenine pathway. Recent crystallographic and biochemical analyses of TDO and IDO have greatly aided our understanding of the mechanisms employed by these enzymes...
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Hemoenzymes are prevalent in nature and participate in a wide range of biological activities. Frequently, high-valence iron intermediates are involved in the catalytic events of these enzymes, especially when the activation of peroxide or dioxygen is involved. Building on the fundamental framework of iron-oxygen chemistry, the mechanistic understandings of these enzymes and their reactive inter...
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In this study, benzoate dioxygenase from Rhodococcus ruber UKMP-5M was catalyzed by oxidating the benzene ring to catechol and other derivatives. The benzoate dioxygenase (benA gene) from Rhodococcus ruber UKMP-5M was then expressed, purified, characterized, The benA gene was amplified (642 bp), and the product was cloned into a pGEM-T vector.The recombinant plasmid pGEMT-benA was digested by d...
متن کاملBPDO biphenyl dioxygenase BphC 2,3-dihydroxybiphenyl 1,2-dioxygenase CPO chloroperoxidase CPR cytochrome P450 oxidoreductase HbpA 2-hydroxybiphenyl 3-monooxygenase NDO naphthalene dioxygenase PAH polycyclic aromatic hydrocarbon PCB polychlorinated biphenyl pNCA p-nitrophenoxy-carboxylic acid TDO toluene dioxygenase TOM toluene ortho-monooxygenase
Oxygenase enzymes have seen limited practical applications because of their complexity, poor stabilities, and often low catalytic rates. However, their ability to perform difficult chemistry with high selectivity and specificity has kept oxygenases at the forefront of engineering efforts. Growing understanding of structure–function relationships and improved protein engineering methods are pavi...
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The EzCatDB (Enzyme Catalytic-mechanism Database) specifically includes catalytic mechanisms of enzymes in terms of sequences and tertiary structures of enzymes, and proposed catalytic mechanisms, along with ligand structures. The EzCatDB groups enzyme data in the Protein Data Bank (PDB) and the SWISS-PROT database with identical domain compositions, Enzyme Commission (EC) numbers and catalytic...
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